Protocol for multi-scale experiments

D41 Protocol for multiscale experimentsTo determine the effects of MNP on soil biota a set of experiments will be performed at three scales Soil biota measurements especially at field level and for specific pot experiments will be supported by soil physicochemical and plant measurements WP5 and MNP levels determination WP61Soil lab experiments no plants 34 soils ca 100 g from soils sampled at CSS in WP3 or characterized control soils in WP5 with variable physicochemical characteristics will be used For each soil three different MNP types selected based on WP3 data to cover 90 of the type of plastics identified in WP3 inventory and CSS applied at rates plus a no plastics control either alone or in combination with three pesticidesveterinary drugs will be tested FUB UTH INRAE and WU will undertake these experiments2Pot experiments coanalysed WP4 5 and 6 34 soils as in lab experiments with plants common with WP5 will be tested 3 different MNP types as in lab experiments applied at 3 rates plus a no plastics control either individually or in combination with three pesticideveterinary drugs will be tested These experiments will be performed by FUB UCSC and UTH questioning effects on soil microbiota and by WU USCS addressing effects on soil fauna3Field experiments coanalysed WP4 5 and 6 3 experimental platforms will be involved Wageningen NL Atlantic region managed by WU Dijon FR Continental region managed by INRAE and Crete EL Mediterranean region managed by CHQ Effects of 2 MNP types will be tested at two rates plus an untreated control in combination or not with one pesticidesveterinary drug in a completely randomized plot design with 3 reps per treatment summing to 24 3 untreated plots Each field experimental site will be supported by at least two groups for the measurements of soil fauna WU UCSC and soil microbiota UTH UCSC CHQ site FUB UTH WU site INRAE FUB INRAE site